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Protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-CT
Federica Orellana1, Annapaola Parrilli1
1Empa - Swiss Federal Laboratories for Materials Science and Technology, 8600 Dübendorf, Switzerland.
Abstract:
Here, we present a protocol for evaluating iodine uptake and diffusion in sheep and pig meniscus tissue using contrast-enhanced micro-computed tomography (CT) and ultraviolet-visible (UV-vis) spectroscopy. We describe steps for sample fixation, a staining procedure with iodine-based contrast agents, and imaging using micro-CT. We then detail procedures for volumetric and radiodensity analyses, UV-vis spectroscopy, and data processing. For complete details on the use and execution of this protocol, please refer to Orellana et al.1.
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