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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Light-sheet Fluorescence Microscopy for the Study of the Murine Heart
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Refractive Index-Corrected Light-Sheet Microscopy for Macro-View Cardiovascular Imaging.

Enbo Zhu1,2,3,4, Yaran Zhang1, Peng Zhao1

  • 1Department of Bioengineering, UCLA, Los Angeles, CA, 90095, USA.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|July 14, 2025
PubMed
Summary
This summary is machine-generated.

A novel refractive index-corrected light-sheet fluorescence microscopy (LSFM) system enables macro-view imaging of cleared organs and organisms. This advanced LSFM system improves resolution and field of view for multi-scale biological imaging.

Keywords:
cardiovascular Imaginglarge field of viewmacro‐view light‐sheet fluorescent microscopyrefractive index correction

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Area of Science:

  • Biomedical Imaging
  • Optical Microscopy
  • Developmental Biology

Background:

  • Light-sheet fluorescence microscopy (LSFM) offers rapid, low-phototoxicity imaging.
  • Optical clearing enhances imaging depth by matching refractive indices (RIs).
  • Existing macro-LSFM systems lack RI correction for diverse clearing methods.

Purpose of the Study:

  • To develop a refractive index-corrected (rc)-LSFM macro-view system for imaging cleared large specimens.
  • To address the challenge of inconsistent RIs caused by various organ-specific clearing protocols.
  • To enable high-resolution, isotropic imaging across multiple scales and biological samples.

Main Methods:

  • Integration of axial sweeping, multi-view imaging, and a closed quartz chamber.
  • Development of a macro-objective system with adjustable refractive index correction.
  • Utilizing diverse optical clearing protocols for cellular to whole-organism samples.

Main Results:

  • Achieved an improved field of view (up to ≈8.8 mm) and spatial resolution (≈3 µm).
  • Demonstrated effective visualization of microvasculature in zebrafish embryos and mouse retinas.
  • Successfully traced cardiomyocyte lineage in mouse embryos and mapped nerve innervation in adult mouse aortas.

Conclusions:

  • The rc-LSFM macro-view system overcomes RI-matching limitations in cleared samples.
  • This technology facilitates multi-scale, high-resolution imaging of complex biological structures.
  • The system supports various clearing protocols for comprehensive cardiovascular and developmental studies.