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4'-hydroxywogonin inhibits oral squamous cell carcinoma progression by targeting Gas6/Axl signaling axis

Tong-Hong Wang1,2,3,4, Li-Fang Chou5, Chin-Chuan Chen1,3

  • 1Biobank, Chang Gung Memorial Hospital, Taoyuan, Taiwan.

Journal of Dental Sciences
|July 14, 2025
PubMed
Abstract

Insights

4'-Hydroxywogonin, a natural compound, effectively inhibits oral squamous cell carcinoma (OSCC) growth and spread. It also enhances cisplatin

Area of Science:

  • Natural Product Chemistry
  • Oncology
  • Molecular Biology

Background:

  • Oral squamous cell carcinoma (OSCC) presents significant therapeutic challenges due to limited efficacy and drug resistance.
  • Natural compounds are being explored for novel anticancer strategies against OSCC.

Purpose of the Study:

  • To investigate the anticancer potential of 4'-hydroxywogonin, a flavonoid from Scutellaria baicalensis, in OSCC.
  • To elucidate the underlying molecular mechanisms of 4'-hydroxywogonin's action in OSCC.

Main Methods:

  • Treatment of OSCC cell lines with 4'-hydroxywogonin.
  • Evaluation of anticancer effects using cell functional assays.
  • Western blot analysis and rescue assays to determine molecular pathways.

Main Results:

  • 4'-Hydroxywogonin selectively inhibited OSCC cell proliferation, migration, and invasion without affecting normal cells.
  • It downregulated Axl/Gas6 signaling, suppressed PI3K/AKT pathway, cell cycle regulators, and EMT-related proteins.
  • 4'-Hydroxywogonin induced G1 phase cell cycle arrest and apoptosis, and enhanced cisplatin's inhibitory effects on OSCC.

Conclusions:

  • 4'-Hydroxywogonin demonstrates significant potential as a novel therapeutic agent for OSCC.
  • Its ability to enhance cisplatin efficacy suggests a promising role in combination therapy for OSCC treatment.
Keywords:
4′-hydroxywogoninAxlGas6Oral squamous cell carcinoma (OSCC)Receptor tyrosine kinases

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