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Updated: Sep 15, 2025

Author Spotlight: Transmitochondrial Cybrid Generation Using Cancer Cell Lines
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Author Spotlight: Transmitochondrial Cybrid Generation Using Cancer Cell Lines

Published on: March 17, 2023

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Mitochondrial Translation Inhibition Uncovers a Critical Metabolic-Epigenetic Interface in Renal Cell Carcinoma

Kazumi Eckenstein1, Beyza Cengiz1, Matthew E K Chang2

  • 1Knight Cancer Institute, Oregon Health and Science University, Portland, OR 97201, USA.

Biorxiv : the Preprint Server for Biology
|July 14, 2025
PubMed
Abstract

Insights

Tigecycline targets mitochondrial translation in renal cell carcinoma (RCC), reducing tumor growth. Combining tigecycline with entinostat shows significant synergy, offering a new therapeutic strategy for RCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Renal cell carcinoma (RCC) presents unique metabolic vulnerabilities.
  • Targeting these vulnerabilities offers therapeutic potential.

Purpose of the Study:

  • Investigate tigecycline's effect on RCC cells.
  • Explore combinatorial therapies to enhance tigecycline efficacy.

Main Methods:

  • Utilized metabolomic profiling, subcellular proteomics, and functional assays.
  • Assessed tigecycline effects in RCC cell lines, organoids, and xenografts.
  • Evaluated tigecycline and entinostat synergy.

Main Results:

  • Tigecycline inhibited mitochondrial translation and reduced RCC tumor growth.
  • Tigecycline induced histone variant accumulation and cell cycle arrest.
  • Combined tigecycline and entinostat demonstrated significant synergism.

Conclusions:

  • Targeting mitochondrial-epigenetic crosstalk is a promising RCC strategy.
  • Combination therapy holds potential for clinical translation.

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