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Updated: Sep 15, 2025

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
05:06

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

741

Genomic Diversity and Antimicrobial Resistance of Acinetobacter sp.-A Pan Genome Analysis

R Shobana Manoharan1, K Raghavan2, M Jayalakshmi1

  • 1Department of Immunology, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625021 India.

Indian Journal of Microbiology
|July 14, 2025
PubMed

Insights

Genomic analysis reveals that Acinetobacter species share virulence and antibiotic resistance genes. Differentiating between Acinetobacter species is crucial for accurate diagnosis and treatment of infections.

Area of Science:

  • Genomics
  • Microbiology
  • Infectious Diseases

Background:

  • Acinetobacter baumannii is a significant cause of hospital-acquired infections.
  • Non-baumannii Acinetobacter species are increasingly implicated in clinical diseases.
  • Understanding the genetic basis of virulence and resistance across Acinetobacter species is essential.

Purpose of the Study:

  • To investigate the genomic linkage of virulence and antibiotic resistance among five Acinetobacter species.
  • To compare the genetic profiles of Acinetobacter baumannii, A. haemolyticus, A. johnsonii, A. nosocomialis, and A. calcoaceticus.

Main Methods:

  • Pan-genome analysis was employed to explore the genomic content of the Acinetobacter species.
  • Resistance gene identification was performed using specialized bioinformatics tools.

Main Results:

  • An open pan-genome was observed in Acinetobacter species, indicating a high degree of genetic variability.
  • Acinetobacter baumannii exhibited resistance to a wide range of antibiotics, including macrolides, fluoroquinolones, and carbapenems.
  • Non-baumannii species showed varying resistance profiles, with A. haemolyticus possessing the Oxa beta-lactamase gene and A. nosocomialis having fluoroquinolone resistance genes. The AbaQ gene was found in A. calcoaceticus and A. nosocomialis, suggesting horizontal gene transfer.

Conclusions:

  • The study highlights the potential for gene transfer of antibiotic resistance among Acinetobacter species.
  • Distinguishing between different Acinetobacter species based on their genetic profiles is critical for effective clinical management.
  • Accurate diagnosis and targeted antimicrobial therapy are necessary for combating Acinetobacter-associated infections.
Keywords:
Acinetobacter sp.BPGAMulti drug resistancePan genome analysis

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