Related Experiment Video
Updated: Sep 15, 2025

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
Effectiveness of Microwave Disinfection on Gutta Percha Cones Against Staphylococcus Aureus and Enterococcus Faecalis
Soundarya Devaraj1, Swetha Venkatakrishnan1, Shreenijha Ramkumar1
1Department of Conservative Dentistry and Endodontics, Sri Ramachandra Dental College and Hospital, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India.
Background:
Non-metallic dental materials like Gutta percha cones, ceramic crowns, endodontic posts are widely used material for the obturation of root canals, placement of post, crown and it is usually distributed by the manufacturers in sealed containers. Once exposed to dental environment or by handling and storage, there are more chances of getting contaminated by various microorganisms. The success of endodontic therapy depends on maintaining the aseptic condition throughout the procedure. To maintain an aseptic condition during endodontic procedures, chairside disinfection of endodontic material is more important. The aim of the study is to evaluate rapid and effective methods of disinfection of non-metallic dental materials using microwave against test organisms.
Materials And Methods:
A total of 30 sample sizes with 5 per group consisting of gutta percha cones of size #30/0.09 taper were used and contaminated with Enterococcus faecalis and Staphylococcus aureus respectively. Further it was disinfected with 3% sodium hypochlorite (NaOCl), 3% NaOCl under microwave, and Distilled water under microwave for 30 sec and 1 min, respectively. The disinfected Gutta percha cones were evaluated by subculture and quantification of colonies formed.
Conclusion:
Microwave disinfection proved to be as effective as 3% and 5% Sodium hypochlorite against Staphylococcus aureus and Enterococcus faecalis. Hence, it proves to be a potential alternative way to disinfect gutta percha cones.
More Related Videos
Related Concept Videos
Antimicrobial Effectiveness
Physical Methods for Controlling Microbial Growth: Radiation and Filtration
Physical Methods for Controlling Microbial Growth: Temperature
Methods of Sterilization I: Physical Methods
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Methods for Controlling Microbial Growth
Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...

