Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

1.9K
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
1.9K
Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

369
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
369
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

3.9K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

4.0K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
4.0K
Microtubule Formation01:23

Microtubule Formation

6.0K
Microtubules are dynamic structures that undergo continuous assembly and disassembly. They originate from specialized multi-protein complexes known as microtubule organizing centers or MTOCs. Within the MTOC, the point of origin of the microtubule is known as the minus end, while the end radiating outward is the plus end. Microtubules serve two primary functions — the organization of spindle complexes to separate sister chromatids during mitotic or meiotic cell division and the formation...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure of the bacteriophage PhiKZ non-virion RNA polymerase bound to a p119L open promoter analogue.

IUCrJ·2025
Same author

Chimeric infective particles expand species boundaries in phage-inducible chromosomal island mobilization.

Cell·2025
Same author

Structure of the Bacteriophage PhiKZ Non-virion RNA Polymerase Transcribing from its Promoter p119L.

Journal of molecular biology·2024
Same author

A novel method for synthesis of carbon dots and their applications in reactive oxygen species (ROS) and glucose detections.

RSC advances·2023
Same author

A 3D-printed flow-cell for on-grid purification of electron microscopy samples directly from lysate.

Journal of structural biology·2023
Same author

Alternative ATPase domain interactions in eukaryotic Hsp70 chaperones.

Frontiers in molecular biosciences·2023

Related Experiment Video

Updated: Sep 15, 2025

Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin
08:07

Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin

Published on: March 24, 2023

2.0K

New insights into tuberous sclerosis complex: from structure to pathogenesis.

Chao-Sheng Chen1, Christopher H S Aylett1

  • 1Section for Structural and Synthetic Biology, Department of Infectious Disease, Imperial College London, London, United Kingdom.

Frontiers in Cell and Developmental Biology
|July 14, 2025
PubMed
Summary

Tuberous sclerosis complex (TSC) arises from mutations in TSC1 and TSC2 genes, impacting cell growth regulation. This review covers the TSC protein complex

Keywords:
GAPRHEBTBC1D7TSChamartinmTORC1rapamycintuberin

More Related Videos

Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles
07:54

Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles

Published on: November 5, 2020

5.3K
Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues
07:21

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

Published on: November 17, 2023

2.2K

Related Experiment Videos

Last Updated: Sep 15, 2025

Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin
08:07

Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin

Published on: March 24, 2023

2.0K
Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles
07:54

Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles

Published on: November 5, 2020

5.3K
Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues
07:21

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

Published on: November 17, 2023

2.2K

Area of Science:

  • Genetics and Molecular Biology
  • Cellular Biology
  • Oncology

Background:

  • Tuberous sclerosis complex (TSC) is a genetic disorder causing benign tumor formation in various organs.
  • Pathogenic variants in the TSC1 and TSC2 tumor suppressor genes are the primary cause of TSC.
  • The TSC1 and TSC2 genes encode hamartin and tuberin, which form a protein complex crucial for regulating cell growth.

Purpose of the Study:

  • To review recent advancements in understanding the molecular structure and function of the TSC protein complex.
  • To elucidate the role of the TSC complex in cellular processes and disease pathogenesis.
  • To summarize current and emerging therapeutic strategies for TSC.

Main Methods:

  • Literature review of recent scientific publications.
  • Analysis of molecular and cellular studies on the TSC complex.
  • Synthesis of information on TSC pathogenesis and therapeutic interventions.

Main Results:

  • The TSC protein complex, comprising hamartin, tuberin, and TBC1D7, is a key regulator of cell growth and proliferation.
  • Dysregulation of the TSC complex impacts cellular processes via mTOR signaling and other pathways.
  • Recent research has deepened the understanding of the complex's structure-function relationship and its role in disease.

Conclusions:

  • The TSC protein complex is central to TSC pathogenesis.
  • Continued research into the complex's function is vital for developing effective therapies.
  • Targeting the TSC complex and its downstream pathways offers promising therapeutic avenues for TSC patients.