Association of ALDH3A1 expression with tumor differentiation, pathological stage, and nodal status in oral squamous cell carcinoma
Related Experiment Video For ALDH3A1 protein
Updated: Jul 4, 2026
Isolation and Characterization of a Head and Neck Squamous Cell Carcinoma Subpopulation Having Stem Cell Characteristics
Published on: May 11, 2016
Rida Fatima Khan1, Shazia Akbar Ansari2, Uzma Bukhari3
1Department of Oral Pathology, Dow Dental College, Dow University of Health Sciences, Pakistan.
Objective:
To observe the association between Aldehyde dehydrogenase 3 family member A1 (ALDH3A1) levels with histological grade, pathological stage and nodal status in Oral Squamous Cell Carcinoma (OSCC).
Methods:
A total of 114 diagnosed OSCC patients who underwent Excisional biopsy with complete neck dissection were recruited in the study in accordance with inclusion criteria. H & E slides were assessed for evaluation of clinicopathological features. Immunohistochemical (IHC) staining method was used to study the expression of ALDH3A1. Statistical analysis was performed using SPSS version 21.0. The chi-square test and Fisher exact test were applied to evaluate the association of ALDH3A1 expression with histological grade, nodal metastasis and pathological tumor stage (pT). All tests were two-sided and a p-value of <0.05 was considered significant.
Results:
This study looked at 114 OSCC patients, most of them were men (76.3 %) with a mean age of 47. The common tumor sites were buccal mucosa (53.5 %) and left side predominance of face (52.6 %). The grades were well (30.7 %), moderate (38.6 %), and 3 poorly differentiated (30.7 %). The expression of ALDH3A1 is significantly strong in the well differentiated compared to the poorly differentiated OSCC (p < 0.001), and presence of nodes involvement compared to no node's involvement (p < 0.001). The findings also suggest that ALDH3A1 expression decreases in more advanced tumors (pT4 stage, p = 0.012), with higher ALDH3A1 levels being associated with earlier stages (pT1, pT2) of OSCC.
Conclusions:
The decrease in expression of ALDH3A1 in a poorly differentiated and advanced stage OSCC compared to its well differentiated and early stage highlights the potential role of this biomarker as an important prognostic tool.
