Related Experiment Video
Updated: Sep 15, 2025

Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection
Published on: September 18, 2020
RCN1 affects malignant progression and macrophage M2 polarization in diffuse large B-cell lymphoma
1College of Medical Technology, Guangxi Health Science College, Nanning, 530021, China.
Reticulocalbin 1 (RCN1) promotes diffuse large B-cell lymphoma (DLBCL) malignancy by enhancing cancer cell growth and M2 macrophage polarization. Inhibiting RCN1 offers a potential therapeutic strategy for DLBCL treatment.
Area of Science:
- Oncology
- Immunology
- Molecular Biology
Background:
- Diffuse large B-cell lymphoma (DLBCL) treatment failure is linked to cancer cell malignancy and macrophage immune evasion.
- Reticulocalbin 1 (RCN1) is implicated in various cancers, but its role in DLBCL is unknown.
Purpose of the Study:
- To investigate the role of RCN1 in the malignant progression of DLBCL.
- To explore RCN1's impact on cancer cell behavior and tumor-infiltrating macrophages in DLBCL.
Main Methods:
- Bioinformatic analysis of RCN1 expression in DLBCL patient data.
- In vitro experiments involving RCN1 knockdown in DLBCL cells.
- Assessment of macrophage polarization (M2 markers) and PI3K/AKT signaling pathway activation.
Main Results:
- High RCN1 expression correlates with poor survival in DLBCL patients.
- RCN1 knockdown reduces DLBCL cell viability and invasion while increasing apoptosis.
- RCN1 promotes M2 macrophage polarization and PI3K/AKT pathway activation, which can be reversed by RCN1 inhibition.
Conclusions:
- RCN1 drives DLBCL progression by directly affecting cancer cells and indirectly influencing M2 macrophage polarization.
- Targeting RCN1 presents a potential therapeutic avenue for treating DLBCL.
More Related Videos
06:38Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line
Published on: August 2, 2021
07:44Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports
Published on: November 28, 2019
Related Concept Videos
Abnormal Proliferation
Differentiation of Common Myeloid Progenitor Cells
Receptor Downregulation in MVBs
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The Tumor Microenvironment