Rapid and efficient low-temperature nitrate reduction in aquaculture wastewater by a recombinant nitrate reductase:
Furong Wang1, Jiayu Ren1, Xin Wang1
1College of Fisheries and Life Science, National Demonstration Center for Experimental Aquaculture Education (Dalian Ocean University), Ministry of Education, Dalian 116023, China; Key Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian 116023, China; Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China.
Abstract:
Heterotrophic nitrification aerobic denitrification represents an exceptionally efficient biological process for nitrogen removal, where nitrate reductase plays a pivotal role as the initial step. Using a crude enzyme solution, AtNR01 was able to degrade 80 % of 1 mg/mL NO3--N within 20 min. Notably, AtNR01 can maintain robust performance over a wide range of temperatures and pH levels. In addition, when AtNR01 and its engineering bacteria are applied to the treatment of aquaculture wastewater, the degradation rate of NO3--N can reach about 85 % within 10 h under the condition of low temperature 10 °C. Molecular docking results indicated that Nitrate binds to mvrA by the amino acid residues Ser 68 A and Phe 81 A and forms hydrogen bonds that help improve the stability of binding. These findings underscore the significant application potential and economic value of AtNR01 and its engineered counterpart in the treatment of low-temperature aquaculture wastewater.
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