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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
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RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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Fast, cost-effective and flexible DNA sequencing by roll-to-roll fluidics.

Yanzhe Qin1,2, Stephan A Koehler3, Yunyan Ling4

  • 1BGI Research, Shenzhen, China. yanzheqin@126.com.

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|July 14, 2025
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Next-generation sequencing (NGS) performance is enhanced using roll-to-roll fluidics (r2r-fl) based on Couette flow. This cost-effective method significantly reduces reagent use and sequencing time for applications in diagnostics and genetic profiling.

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Area of Science:

  • Biotechnology
  • Genomics
  • Fluid Dynamics

Background:

  • Next-generation sequencing (NGS) has revolutionized biological research and clinical diagnostics.
  • Performance improvements in NGS have plateaued due to limitations of Poiseuille fluid dynamics in flow cells.

Purpose of the Study:

  • To overcome limitations in NGS performance by implementing Couette flow.
  • To introduce a cost-effective and scalable NGS method using roll-to-roll fluidics (r2r-fl).

Main Methods:

  • Developed NGS by roll-to-roll fluidics (r2r-fl), a practical implementation of plane Couette flow.
  • Utilized flexible biochip sizes for compatibility and cost-effectiveness.

Main Results:

  • Achieved up to 85-fold lower reagent consumption (US$0.16 per gigabase pair).
  • Reduced rinsing times to under 2 seconds and sequencing turnaround from days to <12 hours.
  • Maintained high accuracy: >99.9% precision for single nucleotide polymorphisms (SNPs), 99.9% mapping rate for E. coli, and minimal errors for SARS-CoV-2.

Conclusions:

  • r2r-fl offers a significant advancement in NGS technology by overcoming fluid dynamic limitations.
  • The method's reduced cost and time enable rapid, scalable applications in pathogen detection, cancer diagnostics, and genetic disease profiling.