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Related Concept Videos

Genome-wide Association Studies-GWAS01:11

Genome-wide Association Studies-GWAS

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Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Updated: Sep 15, 2025

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
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Identification of the Novel HLA-DPA1*02:113 Allele Using Next-Generation Sequencing.

Junhyup Song1, Jongseon Park1, Sunmi Jung2

  • 1Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea.

HLA
|July 15, 2025
PubMed
Summary

A novel Human Leukocyte Antigen (HLA) allele, HLA-DPA1*02:113, has been identified. This new allele is distinguished from a known allele by a single nucleotide difference in exon 1.

Keywords:
HLA‐DPA1*02:113HLAnew allelenext‐generation sequencing

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Area of Science:

  • Immunogenetics
  • Molecular biology
  • Human Leukocyte Antigen (HLA) system

Background:

  • The Human Leukocyte Antigen (HLA) system plays a critical role in immune response and transplantation.
  • Accurate HLA typing is essential for matching donors and recipients and understanding immune-related diseases.

Purpose of the Study:

  • To report the discovery and initial characterization of a novel HLA-DPA1 allele.
  • To provide precise molecular details of the new allele for genetic databases.

Main Methods:

  • High-resolution DNA sequencing of the HLA-DPA1 gene.
  • Bioinformatic analysis to compare the novel sequence with existing HLA allele databases.

Main Results:

  • A new allele, designated HLA-DPA1*02:113, was identified.
  • This allele differs from HLA-DPA1*02:02:02:01 by a single nucleotide substitution at codon-3 in exon 1.

Conclusions:

  • The identification of HLA-DPA1*02:113 expands the known diversity within the HLA-DPA1 locus.
  • This finding contributes to the ongoing efforts to comprehensively catalogue human HLA genetic variation.