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Multivariate Effects of SNPs on Environmental Streptococcal Mastitis Evaluated With an NGS-Based Association Study
Fumihiro Nagata1, Chieh-Wen Lo1,2, Susumu Saito1,2,3
1Laboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
HLA
|July 15, 2025
Summary
Researchers identified genetic markers in the cattle major histocompatibility complex (BoLA) region associated with environmental streptococcal mastitis. This discovery aids in distinguishing mastitis-resistant and susceptible cattle, offering new insights for disease control strategies.
Area of Science:
- Animal Genetics
- Veterinary Immunology
- Bovine Diseases
Background:
- Mastitis, an inflammatory udder condition in cattle, is often linked to bacterial infections.
- The cattle major histocompatibility complex (BoLA) region is implicated in mastitis susceptibility.
- A genetic analysis specifically for environmental streptococcal mastitis was previously lacking.
Purpose of the Study:
- To identify genetic susceptibility markers within the BoLA region for environmental streptococcal mastitis using next-generation sequencing (NGS).
- To conduct a comprehensive genetic analysis of mastitis caused by environmental streptococci in Holstein cattle.
- To discover novel single nucleotide polymorphisms (SNPs) associated with mastitis resistance and susceptibility.
Main Methods:
- Resequencing of the BoLA region using hybridization capture and targeted NGS.
- Variant detection and quality control in 297 Holstein cattle (75 mastitis cases, 222 healthy controls).
- Association study to identify significant SNPs and candidate genes, followed by gene network analysis.
Main Results:
- Detected 2,920,355 variants within the BoLA region.
- Identified five candidate genes (POU5F1, IER3, GNL1, ABCF1, PRR3) associated with mastitis.
- Confirmed significant correlation of six SNPs within these genes for classifying mastitis-resistant and susceptible cattle.
Conclusions:
- This study is the first to report SNPs associated with environmental streptococcal mastitis using NGS-based association analysis in the BoLA region.
- The identified genetic markers are valuable for differentiating mastitis-resistant and susceptible cattle.
- Understanding these host genetic factors provides crucial insights for developing effective mastitis control strategies in cattle.
Keywords:
BoLANGSassociation studybovineenvironmental streptococcimajor histocompatibility complex (MHC)mastitistargeted resequencingMore Related Videos
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