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Targeting Intracellular miRNA in Different Cancer Cell Models Using Gold Nanoprobes and Combined Mass Cytometry and

Sara González-Morales1,2, Lena Schlautmann3, Paula Díez4

  • 1Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain.

Nano Letters
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Summary

DNA-conjugated gold nanoparticles effectively target intracellular microRNA-16-5p in cancer cells. Mass cytometry quantifies nanoprobes, enabling precise measurement of microRNA levels for therapeutic development.

Keywords:
SC-ICP-MSgold nanoprobesmass cytometry (CyTOF)miRNA

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Molecular Biology

Background:

  • MicroRNAs (miRNAs) play crucial roles in cancer development and progression.
  • Targeting specific miRNAs offers a promising strategy for cancer therapy.
  • Developing efficient and quantifiable methods for intracellular miRNA detection is essential.

Purpose of the Study:

  • To develop DNA-conjugated gold nanoparticles (AuNPs) for targeting intracellular miRNA-16-5p in cancer cells.
  • To establish a mass spectrometry-based platform for quantitative evaluation of nanocarrier-mediated miRNA targeting.

Main Methods:

  • AuNPs conjugated with DNA probes were designed to target miRNA-16-5p via base pairing.
  • Multiparametric mass cytometry (CyTOF) was used to quantify Au-nanoprobe uptake in various cancer cell lines.
  • A secondary capture method using magnetic microparticles quantified intracellular miRNA-16-5p levels.

Main Results:

  • Au-nanoprobe uptake varied significantly across cancer cell models, with higher incorporation in lung (A549) and melanoma (A375) cells.
  • CyTOF successfully discriminated Au nanoprobes in intact cells versus cellular debris.
  • The developed platform enabled quantitative assessment of intracellular miRNA-16-5p concentrations.

Conclusions:

  • The study presents a novel platform for quantitative evaluation of nanocarrier-mediated miRNA targeting.
  • This approach facilitates the development of miRNA-based cancer therapeutics.
  • The findings highlight the potential of DNA-AuNPs for targeted intracellular miRNA delivery and quantification.