Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antimicrobial Proteins01:23

Antimicrobial Proteins

5.8K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
5.8K
Selectins01:25

Selectins

3.4K
Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chemical Probes to Reveal the Assembly and Dynamics of Wall Teichoic Acids.

Journal of the American Chemical Society·2026
Same author

Aerocavin Is an Antibiotic with Potent and Specific Anti-Neisserial Activity.

ACS infectious diseases·2026
Same author

Exome sequencing directly implicates 68 genes in inflammatory bowel disease.

medRxiv : the preprint server for health sciences·2026
Same author

It's not just snot! : Mucus: Nature's Multifaceted Secretion.

EMBO reports·2026
Same author

Aerocavin is an antibiotic with potent and specific anti-Neisserial activity.

bioRxiv : the preprint server for biology·2026
Same author

Microbiota-mediated induction of beige adipocytes in response to dietary cues.

Nature·2026
Same journal

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026
Same journal

Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

bioRxiv : the preprint server for biology·2026
Same journal

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026
Same journal

Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

bioRxiv : the preprint server for biology·2026
Same journal

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026
Same journal

Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Sep 15, 2025

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

9.1K

Intelectin-2 is a broad-spectrum antimicrobial lectin.

Amanda E Dugan1,2, Deepsing Syangtan1,2, Eric B Nonnecke3

  • 1Department of Chemistry, Massachusetts Institute of Technology; Cambridge, MA, USA.

Biorxiv : the Preprint Server for Biology
|July 15, 2025
PubMed
Summary
This summary is machine-generated.

Mammalian intelectin-2 binds and crosslinks mucins and microbes. This lectin exhibits antimicrobial activity, crucial for mucosal defense and regulating gut and respiratory microbial communities.

More Related Videos

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

15.8K
Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance
09:59

Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance

Published on: July 21, 2023

1.3K

Related Experiment Videos

Last Updated: Sep 15, 2025

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

9.1K
Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

15.8K
Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance
09:59

Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance

Published on: July 21, 2023

1.3K

Area of Science:

  • Microbiology
  • Immunology
  • Glycobiology

Background:

  • Mammals meticulously manage resident microbiota at mucosal surfaces.
  • Lectins at these sites modulate microbial populations, yet specific functions remain elusive.
  • Intelectins, present in chordates at mucosal barriers, have poorly understood roles.

Purpose of the Study:

  • To characterize the function of mouse intelectin-2 (mItln2) and human intelectin-2 (hItln2) in mucosal defense.
  • To investigate the interaction of intelectins with mucins and microbes.
  • To elucidate the antimicrobial mechanisms of intelectin-2.

Main Methods:

  • Carbohydrate recognition assays to assess intelectin-mucin and intelectin-microbe interactions.
  • Analysis of microbial communities from respiratory and gastrointestinal tracts.
  • Viability assays for human cells and microbes upon intelectin binding.

Main Results:

  • Mouse intelectin-2 (mItln2) and human intelectin-2 (hItln2) bind and crosslink mucins through carbohydrate recognition.
  • Both lectins recognize and bind diverse microbes, including gram-positive and gram-negative bacteria.
  • Intelectin-2 binding leads to microbial death, while human cells remain unaffected.
  • Intelectin-2 engages both host glycans in mucus and microbial surface glycans via calcium-dependent binding.

Conclusions:

  • Mammalian intelectin-2 plays a dual role in mucosal defense: offensive (microbial killing) and defensive (mucus crosslinking).
  • Intelectin-2 is critical for regulating microbial colonization at mucosal barriers.
  • These findings highlight intelectin-2 as a key component of the innate immune system at mucosal surfaces.