Golgi-targeted ratiometric fluorescent probe: Precise detection of H₂S level fluctuations under stress conditions and...

Hydrogen sulfide (H₂S), as an important endogenous gas messenger molecule, changes in its concentration are closely related to oxidative stress and inflammatory responses of cells, which makes H₂S play a key regulatory role in acute liver injury. In order to precisely track the dynamic changes of H₂S in a liver injury model, a novel ratiometric fluorescent probe, NF-FOH, was developed in this study based on aminoquinoline derivatives, which possesses excellent assay properties, such as high sensitivity (10.4 nM), high specificity, and fast response (10 min), as well as Golgi targeting properties and favorable biosafety. These properties enable NF-FOH to achieve portable detection of H₂S via test strips and localization analysis of H₂S in the Golgi. In addition, NF-FOH can effectively detect endogenous H₂S in aspirin-induced and ferroptilized HeLa cells, and can be used to monitor the fluctuation of H₂S levels in cellular, zebrafish, and mouse models of acute liver injury, which provides a powerful tool for in-depth study of the mechanism of H₂S in cellular physiology and pathological processes.