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Host transcriptome profiling for resistance against lumpy skin disease (LSD).

Mohammad Hossein Banabazi1, Steven Van Borm2, Tomas Klingström1

  • 1Department of Animal Biosciences, Swedish University of Agricultural Sciences, Box 7023, Uppsala, 750 07, Sweden.

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|July 15, 2025
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Summary
This summary is machine-generated.

Lumpy skin disease (LSD) affects cattle differently. This study identified Interleukin-1 Receptor Accessory Protein (IL1RAP) gene expression as a key indicator of cattle resilience or susceptibility to LSD virus.

Keywords:
IL1RAP geneCattleDifferentially expressed genes (DEG)Host determinantsLumpy skin disease (LSD)Transcriptome profiling

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Area of Science:

  • Veterinary virology
  • Bovine immunology
  • Transcriptomics

Background:

  • Lumpy skin disease (LSD) is a significant viral disease in cattle.
  • Cattle exhibit varied responses to LSD virus (LSDV) infection.
  • Understanding genetic factors influencing disease resilience is crucial.

Purpose of the Study:

  • To investigate gene expression differences between LSD-resilient and susceptible cattle.
  • To identify potential molecular markers for cattle response to LSDV.
  • To explore the role of specific genes in disease outcome.

Main Methods:

  • Differential gene expression analysis (DEG) in Holstein bulls.
  • Transcriptome profiling before and at three time points after LSDV challenge.
  • Gene Ontology (GO) and KEGG pathway enrichment analysis.

Main Results:

  • Interleukin-1 Receptor Accessory Protein (IL1RAP) gene expression differentiated resilient from susceptible cattle (padj < 0.05).
  • IL1RAP expression shifted from up-regulated pre-infection to down-regulated 3 days post-infection in resilient cattle.
  • Seven days post-infection emerged as a critical time point for LSDV infection dynamics.

Conclusions:

  • IL1RAP gene expression is a potential determinant of cattle resilience to LSDV.
  • This pilot study provides initial insights into bovine host gene expression during LSDV infection.
  • Further research is needed to validate findings and understand mechanisms.