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Related Experiment Video

Updated: Sep 15, 2025

Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs
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Photocrosslinkable Kidney Decellularized Extracellular Matrix-Based Bioink for 3D Bioprinting.

Jaemyung Shin1, Nima Tabatabaei Rezaei2, Subin Choi1

  • 1Department of Biomedical Engineering, Schulich School of Engineering, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.

Advanced Healthcare Materials
|July 16, 2025
PubMed
Summary

Researchers developed a new kidney-specific bioink from decellularized extracellular matrix (KdMA). This biomaterial supports cell viability and enables advanced 3D bioprinting for kidney tissue engineering.

Keywords:
3D bioprintingdecellularized extracellular matrixkidney tissue engineeringphotocrosslinkable bioink

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Three-dimensional bioprinting is crucial for creating tissue constructs for regenerative medicine.
  • Developing organ-specific bioinks that mimic native microenvironments is a significant challenge.
  • Existing bioinks often struggle to support cell viability and tissue maturation.

Purpose of the Study:

  • To develop and characterize a novel photocrosslinkable bioink derived from decellularized porcine kidney extracellular matrix (KdMA).
  • To evaluate the suitability of KdMA bioink for bioprinting applications, including cell encapsulation and multilayer constructs.
  • To assess the potential of KdMA for advancing renal tissue engineering and organoid development.

Main Methods:

  • Decellularization of porcine kidney tissue to isolate extracellular matrix.
  • Methacrylation of decellularized extracellular matrix to create photocrosslinkable KdMA.
  • Characterization of KdMA rheological properties, including stiffness and photocuring kinetics.
  • Bioprinting of human embryonic kidney cells using KdMA via stereolithography and extrusion methods.
  • Assessment of cell viability, spheroid formation, and structural integrity of printed constructs.

Main Results:

  • KdMA bioink demonstrated favorable rheological properties and rapid photocuring.
  • Encapsulated cells showed high viability and formed multicellular spheroids.
  • The bioink supported stable multilayer bioprinting with tunable mechanical properties and preserved structural integrity.
  • KdMA proved compatible with both digital light processing stereolithography and extrusion-based bioprinting.

Conclusions:

  • KdMA is a promising kidney-specific bioink for tissue engineering applications.
  • The bioink's properties support cell viability, proliferation, and tissue maturation.
  • KdMA offers a versatile platform for developing biomimetic kidney constructs and advancing renal organoid development.