Related Experiment Video
Updated: Sep 15, 2025
Herbal Munziq Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting Inflammation
Published on: January 10, 2025
Apigenin attenuates myocardial ischemia-reperfusion injury through miR-448/SIRT1 axis
Chenchen Tian1, Bo Yu2, Yibo Liu1
1School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
Objectives:
Myocardial ischemia/reperfusion injury (MIRI) is the primary pathological injury following ischemic cardiomyocyte therapy, but there are few effective treatments available for MIRI. Apigenin (API) is an active ingredient of herbal medicine. Our study aims to verify whether API regulates autophagy and apoptosis against MIRI via miR-448/Sirtuin-1 (SIRT1) axis.
Materials And Methods:
MTT, SOD, and LDH assays were used to measure cell viability, oxidative stress injury, and cell damage, respectively. RT-qPCR, western blot, and ELISA were used to measure RNA and protein expression levels.
Results:
Compared with the control group, cell viability and SOD levels in the cells of the OGD/R group were significantly decreased, LDH release in the cells was significantly increased, the level of miR-448 in the cells was significantly increased, the levels of SIRT1 mRNA and protein in the cells were significantly increased, the expression of LCII/I and Bcl-2 proteins in the cells were significantly down-regulated, and the expression of p62, Bax proteins in the cells and caspase-3 protein in the cell supernatant were significantly up-regulated. Compared with the OGD/R group, the above indicators were significantly reversed in the OGD/R+API group and the OGD/R+miR-448 inhibitor group. Compared to the OGD/R+miR-448 inhibitor group, the above indicators were significantly reversed in the OGD/R+miR-448 inhibitor+EX527 (SIRT1 inhibitor) group. Compared to the OGD/R+API group, the above indicators were significantly reversed in the OGD/R+API+miR-448 mimic group, OGD/R+API+EX527 group, and OGD/R+API+CA-5f (autophagy inhibitor) group.
Conclusion:
API regulates autophagy and apoptosis via the miR-448/SIRT1 axis against MIRI.
Insights
Apigenin (API) protects against myocardial ischemia/reperfusion injury (MIRI) by regulating autophagy and apoptosis. This study reveals API
Area of Science:
- Cardiovascular Research
- Molecular Biology
- Cellular Biology
Background:
- Myocardial ischemia/reperfusion injury (MIRI) is a significant clinical challenge with limited therapeutic options.
- Apigenin (API), a natural flavonoid, has demonstrated various pharmacological properties.
- Understanding the molecular mechanisms underlying MIRI is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the protective effects of Apigenin (API) against MIRI.
- To elucidate the role of the miR-448/Sirtuin-1 (SIRT1) axis in API-mediated protection.
- To determine whether API modulates autophagy and apoptosis in the context of MIRI.
Main Methods:
- Cell viability was assessed using MTT assays.
- Oxidative stress and cell damage were evaluated by SOD and LDH assays, respectively.
- Gene and protein expression levels were quantified using RT-qPCR, Western blot, and ELISA.
Main Results:
- Apigenin treatment reversed OGD/R-induced decreases in cell viability and SOD levels, and reduced LDH release.
- API modulated miR-448 and SIRT1 expression, influencing autophagy markers (LCII/I, p62) and apoptosis markers (Bcl-2, Bax, caspase-3).
- Experimental manipulations of miR-448 and SIRT1 confirmed their involvement in API's protective effects.
Conclusions:
- Apigenin exerts protective effects against MIRI.
- The miR-448/SIRT1 axis is a key mediator of Apigenin's action.
- API regulates autophagy and apoptosis through the miR-448/SIRT1 pathway to combat MIRI.
