Meta-Research: Positive genetic interactions have greater scientific impact but are under-represented in the...

Genetic interactions, where the combined effect of perturbing two genes leads to a phenotype that deviates from the expectation based on the effects of the individual perturbations, often provide important information about the functional architecture of biological systems. These interactions are commonly classified as negative or positive based on whether the phenotype of the double mutant is less than or greater than what would be expected based on the single mutant effects. When the trait in question is fitness, systematic studies of pairwise deletions have shown that negative interactions typically link genes with similar functional annotations, while positive interactions typically link genes that are less obviously related and thus often viewed as less informative. However, research in the sociology of science suggests that transformative discoveries often arise from unexpected, cross-domain linkages. To evaluate these competing perspectives, we integrated large-scale genetic interaction data in yeast with literature annotations from the Saccharomyces Genome Database and citation data from iCite. We found that positive genetic interactions are associated with greater scientific impact, as measured by citation metrics, contrary to prevailing assumptions. Nevertheless, despite their greater impact, positive interactions are significantly underrepresented in the scientific literature, suggesting they are frequently overlooked. These findings reveal the underappreciated value of positive genetic interactions and demonstrate the potential of applying sociology-of-science insights to foster biological innovation.