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Variation in complement fixation test results with three Histoplasma capsulatum yeast phase antigens
Journal of Clinical Microbiology
|December 1, 1985
Summary
Comparing Histoplasma capsulatum yeast antigens revealed that the VC strain antigen showed better correlation with the Centers for Disease Control reference antigen (A811) than the GW strain antigen.
Area of Science:
- Medical Mycology
- Immunology
- Diagnostic Serology
Background:
- Histoplasma capsulatum is a fungus causing histoplasmosis.
- Accurate serological diagnosis relies on standardized antigens.
- Different yeast strains of H. capsulatum are used for antigen preparation.
Purpose of the Study:
- To compare the equivalency of three Histoplasma capsulatum yeast antigens.
- To evaluate antigen performance using clinical sera samples.
Main Methods:
- Three H. capsulatum yeast antigens (GW, VC, and CDC A811) were utilized.
- Two hundred serum samples were tested.
- Concurrent testing was performed at the Serology Reference Laboratory and the University of Kentucky Mycoserology Laboratory.
Main Results:
- The VC yeast antigen demonstrated a better correlation with the CDC A811 reference antigen.
- The GW yeast antigen showed a lesser correlation compared to the A811 antigen.
- Equivalency testing highlighted differences in antigen performance.
Conclusions:
- The VC yeast antigen is a more equivalent alternative to the CDC A811 reference antigen.
- Antigen selection impacts the reliability of Histoplasma capsulatum serological testing.
- Further validation of yeast antigens is crucial for accurate histoplasmosis diagnosis.