Chitosan@DOTAGA-based hydrogel: A metal-chelating dressing for the treatment of complex wounds

Complex wounds, whether acute or chronic, often suffer from delayed healing, especially when contaminated with metals. These metals worsen the wound environment by promoting the formation of reactive oxygen species (ROS), sustaining inflammation, and promoting microbial growth. Additionally, some heavy metals are intrinsically toxic, further damaging tissues and hindering repair. In this study, we investigated the potential of a novel chitosan-based swelling hydrogel functionalized with the chelating molecule DOTAGA (chitosan@DOTAGA) as a treatment of complex wounds. By combining the known beneficial properties of chitosan with the enhanced metal-binding ability of DOTAGA, this hydrogel aims to tackle the harmful effects of metals in wounds. In vitro assays demonstrated the hydrogel's biodegradability and potent antimicrobial properties, confirming its suitability as a wound dressing. In vivo studies conducted in rat and porcine wound models reveal the hydrogel's effectiveness and safety in various wound types, showcasing its potential to improve healing outcomes. Our results indicate that chitosan@DOTAGA hydrogel significantly improves healing, reducing inflammation and microbial growth. The hydrogel's ability to modulate the wound environment underscores its promise as an advanced dressing for complex wounds particularly in case of metallic contamination.