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Updated: Sep 15, 2025

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
Published on: March 11, 2020
Cell wall polysaccharides response to cadmium stress and transcriptome analysis in tomato (Solanum lycopersicum)
Min Wang1, Muhammad Haris1, Chao Zhang1
1School of Environmental Science and Engineering, Shaanxi University of Science & Technology, Xi'an 710021, PR China.
Abstract:
Cell wall polysaccharides play a vital role in cadmium(II) (Cd(II)) sequestration in plants, yet the molecular mechanisms governing Cd(II) adsorption and the associated transcriptional networks remain unclear. In tomato (Solanum lycopersicum), Cd(II) stress elevated root pectin, hemicellulose, and cellulose contents by 3.41-, 2.79- and 1.11-fold, respectively, enhancing cell wall Cd(II) fixation. Transcriptomics identified 1147/868 (roots) and 632/562 (leaves) up/down-regulated genes. Key transcription factors (WRKY, MYB, bHLH) orchestrated cell wall biosynthesis enzymes (e.g., pectin methylesterase, cellulose synthase, UDP-glycosyltransferase), driving polysaccharide deposition and cell wall thickening. Cd(II) adsorption by polysaccharide components followed pseudo-second-order kinetics and Langmuir isotherms, involving -OH, CO, and C-O-C functional groups. These findings reveal a coordinated regulatory network mediated by transcription factors, which enhances cell wall biosynthesis for Cd(II) tolerance in tomato.
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