Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Circulating Tumor DNA Assessment of Disease Response in Large B-Cell Lymphoma: Lisocabtagene Maraleucel Versus Autologous Stem Cell Transplantation Standard Therapy.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2026
Same author

Quantifying Proximal Tibial Physeal Injury in Rigid Intramedullary Nailing in Adolescent Patients.

Journal of pediatric orthopedics·2026
Same author

Crystal-facet-directed all-vacuum-deposited perovskite solar cells.

Nature materials·2026
Same author

Transient Unexplained Severe Acute Hyperbilirubinaemia and Cholestasis in a Patient With Hereditary Spherocytosis.

Case reports in hepatology·2026
Same author

Personalized Circulating Tumor DNA (ctDNA) Profiling Enables Superior and Universal Measurable Residual Disease (MRD) Detection in Acute Myeloid Leukemia (AML).

medRxiv : the preprint server for health sciences·2026
Same author

Evaluation of sterility testing procedures for laboratory animal rodent diets.

Veterinary and animal science·2026
Same journal

A Brief Overview of Colitis Cystica Profunda and Potential Animal Models.

Journal of the American Association for Laboratory Animal Science : JAALAS·2026
Same journal

Collaboration between Human and Veterinary Research and Medical Experts Could Provide a Balanced Discussion on the Ethics of Using Animals in Research.

Journal of the American Association for Laboratory Animal Science : JAALAS·2026
Same journal

Analysis of IVC Microenvironments During an Extended Cage-Change Interval in Rats (Rattus norvegicus).

Journal of the American Association for Laboratory Animal Science : JAALAS·2026
Same journal

Letter to the Editor: Contemporary Concerns Regarding Laboratory Animal Feed.

Journal of the American Association for Laboratory Animal Science : JAALAS·2026
Same journal

Machine Learning in Nonhuman Primate Models of Infectious Diseases: Current Applications and Future Perspectives.

Journal of the American Association for Laboratory Animal Science : JAALAS·2026
Same journal

Performance Validated 8-Week Sanitation Interval for Mouse (Mus musculus) Wire-Bar Lids: ATP and CFU Outcomes.

Journal of the American Association for Laboratory Animal Science : JAALAS·2026
See all related articles

Related Experiment Video

Updated: Sep 15, 2025

Diagnosis of Ecto- and Endoparasites in Laboratory Rats and Mice
08:03

Diagnosis of Ecto- and Endoparasites in Laboratory Rats and Mice

Published on: September 6, 2011

41.4K

Rodent Pathogen Detection via Testing of Soiled Nesting Material.

David M Kurtz1, Lisa Angermeier2, Maximilian Chisolm3

  • 1Quality Assurance Laboratory, Comparative Medicine Branch, Division of Intramural Research, National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, North Carolina.

Journal of the American Association for Laboratory Animal Science : JAALAS
|July 17, 2025
PubMed
Summary
This summary is machine-generated.

Detecting common murine pathogens in laboratory mice is crucial. Testing soiled nesting material (Nestlet™) offers a sensitive environmental health monitoring method for early pathogen detection and outbreak prevention.

Keywords:
BAPs, blood agar plates (tryptic soy agar with 5% sheep blood)EDT, exhaust dust testingEHM, environmental health monitoringGLMM, generalized linear mixed modelsIVC, individually ventilated cageMNV, mouse norovirusNIEHS, National Institute of Environmental Health SciencesOP, oropharyngealSBS, soiled bedding sentinelsSFSB, sentinel-free soiled beddingTNA, total nucleic acid

More Related Videos

Nest Building Behavior as an Early Indicator of Behavioral Deficits in Mice
06:11

Nest Building Behavior as an Early Indicator of Behavioral Deficits in Mice

Published on: October 19, 2019

20.2K
Assessing Burrowing, Nest Construction, and Hoarding in Mice
08:23

Assessing Burrowing, Nest Construction, and Hoarding in Mice

Published on: January 5, 2012

32.4K

Related Experiment Videos

Last Updated: Sep 15, 2025

Diagnosis of Ecto- and Endoparasites in Laboratory Rats and Mice
08:03

Diagnosis of Ecto- and Endoparasites in Laboratory Rats and Mice

Published on: September 6, 2011

41.4K
Nest Building Behavior as an Early Indicator of Behavioral Deficits in Mice
06:11

Nest Building Behavior as an Early Indicator of Behavioral Deficits in Mice

Published on: October 19, 2019

20.2K
Assessing Burrowing, Nest Construction, and Hoarding in Mice
08:23

Assessing Burrowing, Nest Construction, and Hoarding in Mice

Published on: January 5, 2012

32.4K

Area of Science:

  • Laboratory animal science
  • Veterinary diagnostics
  • Microbiology

Background:

  • Murine pathogens pose risks to laboratory animal health and research integrity.
  • Preventing pathogen spread and outbreaks is critical in research settings.
  • Sensitive pathogen detection methods are essential for environmental health monitoring.

Purpose of the Study:

  • To evaluate the efficacy of soiled nesting material for detecting high-prevalence murine pathogens.
  • To compare the sensitivity of nesting material testing with direct mouse testing.

Main Methods:

  • Collected soiled nesting material (Nestlet™) from occupied mouse cages.
  • Tested nesting material for nucleic acid of mouse norovirus, Helicobacter spp., and Rodentibacter heylii.
  • Assessed pathogen detection in nesting material after transfer of soiled bedding to naive mice.

Main Results:

  • Nesting material from infected mice consistently tested positive for target pathogens.
  • Pathogen detection in nesting material was more sensitive than direct mouse testing, even after bedding transfer.
  • Particulate material in soiled nesting material effectively captured pathogen nucleic acid.

Conclusions:

  • Soiled nesting material is a highly sensitive method for detecting common murine pathogens.
  • This environmental monitoring technique aids in early detection and prevention of pathogen outbreaks.
  • Nestlet™ testing offers a valuable tool for maintaining rodent health and research reliability.