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The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
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Optimizing human B cell repertoire analyses to interpret clinical data and design sequential HIV vaccines

Milton Maciel1, Lindsey R Baden2, Brian Briney3,4,5,6

  • 1Division of AIDS, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA.

NPJ Vaccines
|July 17, 2025
PubMed
Summary

No abstract available in PubMed .

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The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible...
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