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Updated: Sep 15, 2025

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
Published on: March 11, 2020
Whole genome sequence data of Comamonas sediminis FS4_11, a fumonisin B1-transforming bacterium, using hybrid
Yang Wang1, Mengru Zhao1,2, Zhe Wang1
1Institute of Grain and Oil Quality and Safety, Academy of National Food and Strategic Reserves Administration, Beijing 100037, China.
Abstract:
The genome of Comamonas sediminis FS4_11, a bacterial strain with mycotoxin fumonisin B1 (FB1) transformation capability, was sequenced using Oxford Nanopore Technologies (ONT) and Illumina platforms. The final assembly generated a circular chromosome of 5,148,490 bp with a mean G+C content of 63.74%, representing a contiguous genomic structure. Genome annotation predicted 4565 protein-coding sequences (CDSs), 82 transfer RNAs (tRNAs), 18 ribosomal RNAs (rRNAs; 6 each of 5S, 16S, and 23S rRNA), 1 transfer-messenger RNA (tmRNA), and 8 pseudogenes and other non-coding RNAs. Functional annotation identified 939 potential virulence factors, two putative AdeF-related antibiotic resistance genes, 1486 potential pathogen-host interaction proteins, and a candidate carboxylesterase for FB1 transformation. This dataset primarily aids in identifying potential FB1 detoxification enzyme genes and assessing strain biosafety. It also offers significant reuse potential for comparative genomics and understanding bacterial evolution.
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