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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

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High-resolution quantitative phase imaging via vortex beam speckle illumination.

Shengqiang Zhong¹, Hongwei Zou¹, Chao Hou¹

¹School of Science, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, China.

Biomedical Optics Express

|July 18, 2025

Summary

This summary is machine-generated.

This study presents a new vortex beam speckle imaging system for quantitative phase imaging (QPI). The system significantly enhances lateral resolution and signal-to-noise ratio for precise, label-free biomedical imaging.

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Area of Science:

Optics and Photonics
Biomedical Imaging
Microscopy

Background:

Quantitative Phase Imaging (QPI) is crucial for label-free cell analysis.
Traditional QPI systems face limitations in lateral resolution and signal-to-noise ratio.
Vortex beams offer unique properties for advanced imaging techniques.

Purpose of the Study:

To develop a novel vortex beam speckle imaging system for enhanced QPI.
To improve lateral resolution and signal-to-noise ratio in phase imaging.
To demonstrate the system's capability for precise phase retrieval in biological samples.

Main Methods:

Utilized vortex beams for non-diffracting speckle field regulation.
Implemented a speckle imaging system for quantitative phase imaging.
Applied Transport-of-Intensity Equation (TIE) phase retrieval algorithms.

Main Results:

Reduced speckle size from 116.32 μm to 11.07 μm.
Achieved 1.52-fold improvement in lateral resolution compared to traditional systems.
Increased intensity signal-to-noise ratio from 13.26 dB to 30.62 dB.

Conclusions:

The vortex beam speckle imaging system offers superior lateral resolution and SNR for QPI.
Demonstrated precise phase retrieval using standard targets and red blood cell samples.
The system shows significant potential for label-free, non-invasive biomedical imaging applications.