Analysis of glucose concentrations in blood solutions using FTIR and Raman spectroscopy methods

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Spectroscopy

Background:

• Current invasive blood glucose monitoring methods present challenges including discomfort, time, and cost.
• Non-invasive blood glucose measurement (NGM) is a significant area of research and development.
• Advancements in Fourier transform infrared (FTIR) and Raman spectroscopy offer potential for NGM.

Purpose of the Study:

• To investigate the combined use of FTIR and Raman spectroscopy for non-invasive blood glucose measurement.
• To identify and differentiate molecular bonds between glucose and hemoglobin.
• To analyze spectral variations across the 4000 cm⁻¹ to 400 cm⁻¹ wavenumber range.

Main Methods:

• Utilized a combined approach of FTIR and Raman spectroscopy.
• Analyzed invasive blood samples.
• FTIR spectroscopy was performed on a potassium bromide tablet.
• Raman spectroscopy was conducted on a glass slide.

Main Results:

• Observed distinct variations in atomic types and bonding characteristics within specific wavenumber ranges.
• As blood glucose concentration increased, FTIR absorption intensity and spectrum area decreased.
• Conversely, Raman signal intensity and area under the curve increased with rising blood glucose concentration.

Conclusions:

• The observed spectral changes are linked to hydrogen bond formation and altered scattering properties.
• The study demonstrates the potential of combined FTIR and Raman spectroscopy for NGM.
• This technique may offer a more comfortable and efficient alternative to invasive glucose monitoring.