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Updated: Aug 7, 2026

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
Published on: September 21, 2018
Flagellar morphology in stumpy-flagella mutants of Chlamydomonas reinhardtii
Abstract:
Sixteen new mutants of the biflagellate green alga Chlamydomonas reinhardtii with either stumpy-flagella or no flagella at all were examined by electron microscopy. Four of the mutants were found to carry short bulbous flagella containing amorphous electron-dense material which may represent unassembled flagellar protein. Basal bodies of normal ultrastructure were present in all mutants. Dikaryon dominance tests indicated that the stumpy mutations were recessive to wild-type in all cases tested. Stumpy mutations also conferred a measure of detergent resistance to Chlamydomonas, apparently by affecting the detergent-solubility of the flagellar membrane.
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