New insights into the regulation mechanism of starch digestibility by polyphenol structures in
Ran Meng1, Ran Feng1, Si-Jie Wu1
1Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China; School of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.
Abstract:
The study aimed to investigate the effect mechanism of polyphenol structures on starch digestibility in the starch-polyphenol-protein ternary system. Polyphenol structures affected the affinity between polyphenols and whey protein isolate (WPI), with flavonoid-based polyphenols possessing a higher affinity to WPI. The law of polyphenol structures impacting the starch multiscale structure was altered in starch-polyphenol-protein ternary system compared to starch-polyphenol binary system. Rice starch-genistein-WPI complex and rice starch-caffeic acid-WPI complex demonstrated the highest short-range ordering and long-range ordering, respectively. Quercetin exhibited the highest enzyme inhibitory activity in presence of WPI. Particularly, the rice starch-quercetin-WPI complex showed the lowest starch digestibility (55.7 %) and the highest resistant starch content (46.0 %), likely due to the strongest inhibitory effect of quercetin on amylases. This study provided a theoretical guidance for the development of slow digestible starch-based foods.
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