Related Experiment Videos
Mouse chromosomal mapping of a murine leukemia virus integration region (Mis-1) first identified in rat thymic
Abstract:
We have previously identified a region of genomic DNA which constitutes the site of frequent provirus integration in rat thymomas induced by Moloney murine leukemia virus (Lemay and Jolicoeur, Proc. Natl. Acad. Sci. USA 81:38-42, 1984). This genetic locus is now designated Mis-1 (Moloney integration site). Cellular sequences homologous to Mis-1 are present in mouse DNA. Using a series of hamster-mouse somatic cell hybrids, we mapped the Mis-1 locus to mouse chromosome 15. Frequent chromosome 15 aberrations have been described in mouse thymomas. Mis-1 represents a putative new oncogene which might be involved in the initiation or maintenance or both of these neoplasms.
Insights
Researchers identified the Moloney integration site-1 (Mis-1) in rat thymomas. This gene, located on mouse chromosome 15, is a potential oncogene implicated in thymoma development.
Area of Science:
- Molecular biology
- Oncology
- Genetics
Background:
- Moloney murine leukemia virus (MMLV) frequently integrates into specific genomic regions in induced rat thymomas.
- A previously identified integration site has been designated Moloney integration site-1 (Mis-1).
Purpose of the Study:
- To map the location of the Mis-1 locus in the mouse genome.
- To investigate the potential role of Mis-1 as an oncogene in thymoma development.
Main Methods:
- Utilized hamster-mouse somatic cell hybrids for genetic mapping.
- Analyzed the chromosomal location of the Mis-1 locus.
Main Results:
- The Mis-1 locus was successfully mapped to mouse chromosome 15.
- Cellular sequences homologous to Mis-1 were found in mouse DNA.
- Chromosome 15 aberrations are frequently observed in mouse thymomas.
Conclusions:
- Mis-1 is located on mouse chromosome 15.
- Mis-1 is a candidate oncogene potentially involved in thymoma initiation or maintenance.