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Related Experiment Video

Updated: Jun 25, 2026

Fluorescence Activated Cell Sorting of Plant Protoplasts

Fluorescence Activated Cell Sorting of Plant Protoplasts

Published on: February 18, 2010

Fluorescence-activated particle sorting for condensate purification.

Annie Munier Godebert¹, Dominique Weil^2,3, Adham Safieddine^4,5

¹Research Center Saint-Antoine (CRSA), CISA Flow Cytometry Facility, UMRS 938, Sorbonne University, Paris, France.

Nature Protocols

|July 18, 2025

Summary

This summary is machine-generated.

This study introduces fluorescence-activated particle sorting (FAPS) to purify cellular condensates like P-bodies (PBs). This novel method enables detailed analysis of condensate protein and RNA content, advancing cell biology research.

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Last Updated: Jun 25, 2026

Fluorescence Activated Cell Sorting of Plant Protoplasts

Fluorescence Activated Cell Sorting of Plant Protoplasts

Published on: February 18, 2010

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Cellular condensates organize subcellular space in eukaryotes, including nucleoli, paraspeckles, P-bodies (PBs), and stress granules.
Investigating condensate biology often involves analyzing their protein and RNA content.
Purifying condensates is challenging due to similar densities to other organelles and lack of exclusive markers.

Purpose of the Study:

To present a novel protocol for purifying cellular condensates using fluorescence-activated particle sorting (FAPS).
To enable detailed characterization of the protein and RNA content of purified condensates.
To provide a method adaptable for various condensates across different model organisms.

Main Methods:

Fluorescence-activated particle sorting (FAPS) protocol for condensate purification.
Fluorescent labeling of condensates for identification and isolation.
Focus on purification, quality control, and downstream characterization of P-body (PB) protein and RNA content.

Main Results:

Demonstrated the feasibility of FAPS for isolating PBs from human cell lines.
Established a method for quality control and downstream analysis of PB contents.
Showcased FAPS's potential for characterizing diverse condensates.

Conclusions:

FAPS offers a new approach to overcome limitations in condensate purification.
This method facilitates the characterization of protein and RNA composition in various condensates.
FAPS is adaptable for studying condensate biology across different cell types and organisms.