Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nuclear Overhauser Enhancement (NOE)01:07

Nuclear Overhauser Enhancement (NOE)

834
Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling.  This phenomenon, called the Nuclear Overhauser Enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring...
834
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

298
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
298
Nuclear Magnetic Resonance (NMR): Overview01:07

Nuclear Magnetic Resonance (NMR): Overview

3.5K
Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
3.5K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.1K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.1K
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

296
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
296
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.2K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enabling Quantitative Benchtop <sup>13</sup>C NMR Spectroscopy in Fast Continuous Flow.

Magnetic resonance in chemistry : MRC·2026
Same author

CHAOS - A Large-scale Database for σ-Profiles and Other Molecular Descriptors.

Journal of chemical information and modeling·2026
Same author

Batch Distillation Data for Developing Machine Learning Anomaly Detection Methods.

Scientific data·2026
Same author

Zero- to ultralow-field J-spectroscopy with a diamond magnetometer.

Communications chemistry·2026
Same author

Enabling nondestructive observation of electrolyte composition in batteries with ultralow-field nuclear magnetic resonance.

Chemical science·2026
Same author

Quantum magnetic J-oscillators.

Nature communications·2026
Same journal

Influence of Magnetic Field and Solvent Environment on Laser-Ablated Ag and Cu-Based Nanoparticles: Optical and Thermal Correlations.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

Beyond Conventional Catalyst Design: A Perspective on the Inverse Catalyst Strategy in Ammonia Synthesis and Decomposition.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

A Theoretical Study of Electron Attachment to Uracil and 5-Halouracil.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

A Short Review on the Electron Transfer at the Interface Metal/Semiconductor During Hydrogen Ions Reduction to H<sub>2</sub> Under Photoirradiation.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

Spectroscopic Investigation of the In Vivo Light-Dependent Photodynamics of the Marine Diatom Phaeodactylum tricornutum.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

Atomistic Insights into the Thermal Decomposition and Runaway Mechanism of Peroxypropionic Acid.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
See all related articles

Related Experiment Video

Updated: Sep 14, 2025

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

10.6K

Quantitative Nuclear Magnetic Resonance Spectroscopy with Overhauser Dynamic Nuclear Polarization.

Johnnie Phuong1,2, Raphael Kircher1,2, Sarah Mross1,2

  • 1Laboratory of Engineering Thermodynamics (LTD), RPTU Kaiserslautern, Erwin-Schrödinger-Straße 44, 67663, Kaiserslautern, Germany.

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
|July 19, 2025
PubMed
Summary
This summary is machine-generated.

Overhauser dynamic nuclear polarization (ODNP) enhances nuclear magnetic resonance (NMR) sensitivity for analyzing diluted samples. A new calibration method enables accurate quantitative analysis of mixtures using ODNP-enhanced NMR, overcoming previous limitations.

Keywords:
13C1Hbenchtop NMR spectrometerdynamic nuclear polarizationquantitative analysis

More Related Videos

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

10.8K
High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

5.7K

Related Experiment Videos

Last Updated: Sep 14, 2025

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

10.6K
Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

10.8K
High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

5.7K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Chemical Engineering

Background:

  • Nuclear magnetic resonance (NMR) spectroscopy is valuable for process monitoring but lacks sensitivity for diluted samples.
  • Hyperpolarization techniques like Overhauser dynamic nuclear polarization (ODNP) can significantly enhance NMR signals.
  • ODNP's application to quantitative mixture analysis has been limited due to variable signal enhancements.

Purpose of the Study:

  • To address the challenge of quantitative analysis of mixtures using ODNP-enhanced NMR.
  • To demonstrate the feasibility of ODNP for analyzing highly diluted components in flowing samples.
  • To develop a robust calibration procedure for accurate ODNP-enhanced NMR mixture quantification.

Main Methods:

  • Utilized Overhauser dynamic nuclear polarization (ODNP) with 1 ${^1}$ H and 13 ${^{13}}$ C NMR spectroscopy.
  • Conducted continuous-flow experiments using a benchtop NMR spectrometer.
  • Implemented a new tailored calibration procedure for quantitative analysis of binary mixtures.

Main Results:

  • Achieved significant signal enhancements using ODNP-enhanced NMR spectroscopy.
  • Successfully quantified components in binary mixtures across a wide concentration range.
  • Demonstrated the robustness of the new calibration procedure under challenging conditions.

Conclusions:

  • ODNP-enhanced NMR spectroscopy, combined with a robust calibration, enables quantitative analysis of mixtures.
  • This approach overcomes the sensitivity limitations of traditional NMR for diluted samples.
  • The method is suitable for analyzing flowing samples in continuous-flow experiments.