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Injectable Gellan Gum Hydrogel With Plga-ldh Microspheres For Controlled Alendronate Release And Bone Regeneration.

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Injectable Gellan Gum Hydrogel With Plga-ldh Microspheres For Controlled Alendronate Release And Bone Regeneration.

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Injectable gellan gum hydrogel with PLGA-LDH microspheres for controlled alendronate release and bone regeneration.

Alireza Sedaghat¹, Fatemeh Shokrolahi¹, Hamid Yeganeh²

¹Department of Biomaterials, Faculty of Science, Iran Polymer and Petrochemical Institute, Tehran, Iran.

International Journal of Biological Macromolecules

|July 19, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

This study introduces Gel-PMA, an injectable hydrogel scaffold for bone regeneration. It provides controlled alendronate release and enhanced osteogenic potential, improving bone healing.

Keywords:

Alendronate intercalated AL-Zn-LDH Gellan gum gelatin injectable hydrogel PLGA microsphere Sodium alendronate

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Area of Science:

Biomaterials Science
Tissue Engineering
Drug Delivery

Background:

Bone defects pose significant challenges in regenerative medicine.
Alendronate (ALN) is crucial for bone regeneration but requires effective delivery systems.
Injectable hydrogels offer minimally invasive delivery for bone tissue engineering.

Purpose of the Study:

To develop an injectable Gellan Gum-Gelatin hydrogel (GG/gelatin hydrogel) composite with PLGA-LDH microspheres (Gel-PMA) for controlled alendronate release.
To evaluate the osteogenic potential and mechanical properties of the developed Gel-PMA scaffold for bone regeneration.

Main Methods:

Synthesized Manganese-Zinc Layered Double Hydroxide (LDH) nanoparticles and loaded them with alendronate (ALN-LDH).
Encapsulated ALN-LDH into poly(d,l-lactide-co-glycolide) (PLGA) microspheres using a double-emulsion technique.

Incorporated PLGA microspheres into a GG/gelatin hydrogel, crosslinked with CaCl₂, and characterized the composite (Gel-PMA).

Main Results:

Gel-PMA exhibited enhanced compressive strength (30 to 170 kPa) compared to the hydrogel alone.
Achieved sustained alendronate release (∼95% in 28 days) with improved drug availability.
Demonstrated excellent biocompatibility (>90% cell viability) and significant osteogenic potential in vitro, evidenced by increased ALP activity, calcium deposition, and gene expression (ALP, Col I, OCN).

Conclusions:

Gel-PMA is a promising injectable scaffold for bone tissue engineering.
The composite offers controlled drug release, improved mechanical properties, and superior osteogenic differentiation.
This technology holds potential for enhanced bone regeneration therapies.