Pulp response following direct pulp capping with Tideglusib and mineral trioxide aggregate: an animal study

Area of Science:

• Biomaterials Science
• Dental Research
• Regenerative Medicine

Background:

• Direct pulp capping (DPC) aims to preserve pulp vitality using various biomaterials.
• Mineral trioxide aggregate (MTA) is a commonly used DPC material with variable success.
• Tideglusib, a glycogen synthase kinase-3 inhibitor, is a novel agent explored for DPC.

Purpose of the Study:

• To compare the pulp response to Tideglusib and MTA when used for direct pulp capping.
• To evaluate the histopathological outcomes of DPC with these two materials in a canine model.

Main Methods:

• Class V cavities with pulp exposure were created in 56 canine teeth.
• Teeth were divided into two groups: MTA (ProRoot white MTA) and Tideglusib (50 nM solution on collagen).
• Histopathological evaluations were performed at 3 and 8 weeks, assessing inflammation, tissue disorganization, and calcific bridge formation.

Main Results:

• Tideglusib group showed significantly higher inflammatory cell counts and pulp tissue disorganization compared to the MTA group (P < 0.05).
• No statistically significant difference in new hard tissue (calcific bridge) formation was found between the MTA and Tideglusib groups (P = 0.157).

Conclusions:

• Tideglusib, when used for direct pulp capping, elicits a greater inflammatory response and soft tissue disorganization than ProRoot white MTA.
• Both materials demonstrated comparable capacity for hard tissue formation in this canine model.