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Highly efficient photoelectrochemical biosensor for the evaluation of bisphenol A-induced microRNA-21 expression
Lingling Luo1, Yuying Zhou1, Hongbo Li2
1Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Abstract:
Herein, we developed a photoelectrochemical (PEC) biosensor integrating SrTiO3 and SnO2 materials with a dumbbell DNA mediated target recycling system to quantify microRNA-21 levels under bisphenol A (BPA) exposure. The engineered SrTiO3/SnO2 photoelectrode demonstrated enhanced charge separation efficiency due to band alignment, yielding a remarkable 33-fold photocurrent enhancement compared to that of pure SrTiO3. In addition, a dumbbell DNA-based signal amplification strategy was designed and the target microRNA-21 was converted to DNA nanospheres, which can "switch-on" the PEC signal by loading the signal mediator methylene blue. Through the synergistic optimization of photoelectric performance and signal amplification, the biosensor achieved ultrasensitive microRNA-21 detection with a detection limit of 2.1 fM. The PEC biosensor was employed to monitor the BPA-induced microRNA expression alterations. This study provides a promising paradigm for the construction of PEC biosensors for use in potential environmental toxicological evaluation.
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