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Surface-Enhanced Raman Spectroscopy (SERS) Based Biological and Environmental 2D and 3D Imaging
Qishen Huang1, Huiyuan Guo2, Wei Wang3
1School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.
ACS Environmental Au
|July 21, 2025
Summary
Surface-enhanced Raman spectroscopy (SERS) imaging offers sensitive, real-time analysis for biological and environmental samples. Addressing its challenges will expand SERS applications in diagnostics and contaminant monitoring.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Biotechnology
Background:
- Surface-enhanced Raman spectroscopy (SERS) imaging is a powerful technique for molecular analysis.
- It provides high sensitivity and spatial resolution for in situ detection.
- SERS imaging is valuable in both biological and environmental studies.
Purpose of the Study:
- To review the applications of SERS imaging in biological and environmental fields.
- To highlight advancements in SERS techniques for various analytes.
- To discuss the challenges and future potential of SERS imaging.
Main Methods:
- SERS imaging utilizes plasmonic nanostructures to enhance Raman signals.
- Techniques include nanoassembly, bioorthogonal chemistry, and antibody conjugation.
- Integration with other imaging modalities is employed for in vivo studies.
Main Results:
- SERS imaging successfully detects nucleic acids, proteins, biomarkers, and pathogens in biological systems.
- Environmental applications include tracking pesticides, nanoparticles, and heavy metals.
- SERS-based imaging provides spatial data on pH and reactive oxygen species (ROS).
Conclusions:
- SERS imaging offers sensitive detection and distribution analysis of analytes.
- Current challenges need to be addressed for broader implementation.
- SERS imaging holds significant promise for contaminant monitoring, clinical diagnostics, and biological analysis.
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