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Related Experiment Video

Updated: Sep 14, 2025

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Photodynamically tunable ROS-generating hydrogels for accelerated tissue regeneration.

Seung Hee Hong1,2, Ye Jin Park1, Seo In Lee1

  • 1Department of Medical Engineering, Yonsei University, College of Medicine, Seoul 03722, Republic of Korea.

Bioactive Materials
|July 21, 2025
PubMed
Summary

Reactive oxygen species (ROS)-generating hyaluronic acid (HA) hydrogels accelerate wound healing. These novel HA hydrogels promote cell proliferation, collagen deposition, and angiogenesis for enhanced tissue repair.

Keywords:
AngiogenesisHyaluronic acidHydrogelProliferationReactive oxygen speciesWound healing

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Wound healing involves complex cellular processes like fibroblast and keratinocyte proliferation and angiogenesis.
  • Hyaluronic acid (HA) hydrogels are promising biomaterials for wound healing applications.
  • Controlled generation of reactive oxygen species (ROS) can modulate cellular activities crucial for tissue repair.

Purpose of the Study:

  • To investigate the wound-healing potential of a novel ROS-generating HA hydrogel.
  • To evaluate the effects of ROS generated by a chlorin e6-conjugated HA (Ce6-HA) hydrogel on cellular activities involved in wound repair.
  • To assess the efficacy of Ce6-HA hydrogels in promoting wound closure and tissue regeneration in vivo.

Main Methods:

  • Fabrication of a chlorin e6-conjugated HA (Ce6-HA) hydrogel capable of generating ROS upon LED light irradiation.
  • In vitro assessment of ROS effects on fibroblast and keratinocyte proliferation, intracellular ROS levels, protein expression (p-ERK1/2, p-p38 MAPK, p-Akt, cyclin D1), collagen deposition, and endothelial angiogenesis.
  • In vivo evaluation of Ce6-HA hydrogel efficacy in a wound healing model, including wound closure, tissue regeneration, and histopathological/immunohistochemical analyses.

Main Results:

  • In vitro studies showed that ROS generated by Ce6-HA hydrogels enhanced fibroblast and keratinocyte proliferation.
  • Fibroblasts exhibited increased intracellular ROS, elevated expression of key proteins (p-ERK1/2, p-p38 MAPK, p-Akt, cyclin D1), and augmented collagen deposition.
  • Ce6-HA hydrogels promoted endothelial angiogenesis in vitro and significantly improved wound closure and tissue regeneration in vivo, with enhanced cellular proliferation and angiogenesis.
  • Histopathological analysis revealed elevated levels of critical growth factors and signaling molecules in the Ce6-HA treated group, indicating activated molecular pathways for skin repair.

Conclusions:

  • ROS-generating Ce6-HA hydrogels effectively promote key cellular activities essential for wound healing.
  • Controlled ROS generation by these HA hydrogels activates molecular pathways crucial for skin tissue repair.
  • ROS-triggering HA hydrogels represent a promising therapeutic strategy for accelerating wound recovery and minimizing scarring in clinical settings.