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Key immune regulators in retinal ischemia-reperfusion injury via RNA sequencing
Shan He1, Cui-Ying Liu2, Chang-Hong Ren3
1Department of Ophthalmology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.
International Journal of Ophthalmology
|July 21, 2025
Summary
This study identifies key immune genes, Irf7 and Tap1, involved in retinal ischemia-reperfusion injury (RIRI). These genes in microglia and dendritic cells offer potential therapeutic targets for reducing RIRI inflammation and improving recovery.
Area of Science:
- Ophthalmology
- Immunology
- Molecular Biology
Background:
- Retinal ischemia-reperfusion injury (RIRI) is a significant cause of vision loss.
- Understanding the immune response in RIRI is crucial for developing effective treatments.
Purpose of the Study:
- To investigate immune cell infiltration and molecular mechanisms in RIRI.
- To identify potential therapeutic targets for RIRI.
Main Methods:
- Bulk and single-cell RNA sequencing (RNA-seq) to identify hub genes and cell-specific expression.
- QuanTIseq for immune cell composition analysis.
- Protein-protein interaction and cell-cell communication analyses.
- Quantitative real-time polymerase chain reaction (qRT-PCR) for validation.
Main Results:
- Identified 8 hub genes including Stat2, Irf7, Irgm1, Igtp, Parp9, Irgm2, Nlrc5, and Tap1.
- Found Irf7 predominantly in microglia and Tap1 in dendritic cells (DCs).
- Microglia and DCs play central roles in coordinating immune activity during RIRI.
Conclusions:
- Irf7 and Tap1 are key immune regulators in the acute phase of RIRI.
- Targeting Irf7 and Tap1 may reduce inflammation and promote neurological recovery in RIRI.
