Enhanced production of biodegradable polyhydroxybutyrate PHB plastic through the combination of eco-friendly...

Polyhydroxybutyrate (PHB) is an environmentally friendly polymer that is synthesized by bacteria and stored as a carbon source. One of the main problems in PHB production is the price of materials for production. This study aims to optimize environmentally friendly and affordable materials such as molasses, tofu wastewater, and coconut water to increase PHB yield. Statistical optimization with central composite design (CCD) was used to optimize materials in Bacillus cereus B52 (B. cereus B52) for PHB production. The concentration of molasses, tofu wastewater and coconut water were used as optimization parameters to increase the PHB yield. The optimization results resulted in the production of PHB yield of 1.45 ± 0.14 g/L. Chemical analysis was used to determine the biopolymer validation of PHB. In conclusion, production optimization using a combination of materials from molasses, tofu wastewater, and coconut water can increase PHB production in B. cereus B52.