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Updated: Jul 3, 2026

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Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
Published on: March 25, 2015
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Evaluation of synthetic peptides for direct protein delivery into Saccharomyces cerevisiae
Shota Isogai1, Ryoya Tanahashi2, Hiroshi Takagi1
1Institute for Research Initiatives, Nara Institute of Science and Technology, Nara, Japan.
Bioscience, Biotechnology, and Biochemistry
|July 21, 2025
Abstract:
Efficient intracellular delivery of functional proteins into the yeast Saccharomyces cerevisiae remains a major technical challenge due to its rigid cell wall. Here, we report that synthetic peptide tags, particularly penetratin, enable nearly complete delivery of GFP, albeit with substantial cytotoxicity. This method provides a promising non-genetic platform for the intracellular delivery and functional analysis of proteins in yeast systems.

