Related Experiment Video

Updated: Sep 14, 2025

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
09:40

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

5.7K

A Novel C19orf47-AKT2 Chimeric RNA Generated by Cis-Splicing of Adjacent Genes Is Associated With Glioblastoma

Zihan Wang1,2, Bowen Ni3, Kezhi Wu3

  • 1Department of Neurosurgery, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China.

Annals of Clinical and Translational Neurology
|July 22, 2025
PubMed
Abstract

Insights

Novel chimeric RNAs, C19orf47-AKT2, were found in gliomas and promote tumor growth. These findings suggest C19orf47-AKT2 chimeras could be new biomarkers and therapeutic targets for glioma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Malignant gliomas present significant challenges in treatment.
  • Novel molecular targets are crucial for advancing precision oncology.

Purpose of the Study:

  • To identify and characterize novel chimeric RNAs in glioma.
  • To assess the clinical and functional significance of these chimeras for targeted glioma therapy.

Main Methods:

  • RNA sequencing and polymerase chain reaction (PCR) were used to identify and validate C19orf47-AKT2 chimeric RNAs.
  • Quantitative PCR and Kaplan-Meier analysis assessed expression and prognostic significance in glioblastoma.
  • Genomic analysis, Western blotting, and CCK-8 assays investigated formation mechanisms and functional impact on glioma proliferation.

Main Results:

  • C19orf47-AKT2 chimeric RNAs were detected in 88.9% of glioma core tissues, significantly more than in peritumoral tissues.
  • High expression of the C19orf47e9-AKT2e2 variant correlated with poor glioblastoma prognosis.
  • Downregulation of these chimeras suppressed patient-derived glioblastoma cell proliferation, despite no detected endogenous fusion protein.

Conclusions:

  • Novel C19orf47-AKT2 chimeras, formed via cis-splicing, may function as noncoding RNAs promoting glioblastoma proliferation.
  • These chimeras represent potential prognostic markers and therapeutic targets for glioma treatment.
Keywords:
C19orf47‐AKT2chimeric RNAcis‐splicing of adjacent genes (cis‐SAGes)fusion geneglioma

More Related Videos

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients08:25

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

Published on: September 9, 2020

11.2K
Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing09:49

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing

Published on: July 5, 2019

9.7K

Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
57.1K
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.7K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
5.4K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.1K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
5.1K
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.2K