Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ligand Binding Sites02:40

Ligand Binding Sites

13.3K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
13.3K
Selectins01:25

Selectins

3.4K
Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editor's Choice GlycoEnzDB: a database of enzymes involved in human glycosylation.

Glycobiology·2025
Same author

GlycoEnzDB: A database of enzymes involved in human glycosylation.

bioRxiv : the preprint server for biology·2025
Same author

Engineering glycosyltransferases into glycan binding proteins using a mammalian surface display platform.

Nature communications·2025
Same author

Mammalian Cell Surface Display for High-Throughput Protein Engineering of Glycosyltransferases.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Tuning the tropism and infectivity of SARS-CoV-2 virus-like particles for mRNA delivery.

Nucleic acids research·2025
Same author

Cell- and tissue-specific glycosylation pathways informed by single-cell transcriptomics.

NAR genomics and bioinformatics·2024

Related Experiment Video

Updated: Sep 14, 2025

Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System
05:19

Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System

Published on: June 28, 2024

987

Editor's Choice Protein engineering strategies to develop lectins by design.

Ryoma Hombu1, Lauren E Beatty2, Sriram Neelamegham1,2,3

  • 1Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY 14260, USA.

Glycobiology
|July 22, 2025
PubMed
Summary

Developing novel glycan-binding proteins (GBPs) is crucial for understanding cell biology. This review details protein engineering strategies, including rational design and directed evolution, to create advanced GBPs for glycomics research.

Keywords:
directed evolutionglycanlectinmutagenesissurface display

More Related Videos

Protein Engineering by Yeast Surface Display
05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

1.9K
Design and Construction of Artificial Extracellular Matrix aECM Proteins from Escherichia coli for Skin Tissue Engineering
10:30

Design and Construction of Artificial Extracellular Matrix aECM Proteins from Escherichia coli for Skin Tissue Engineering

Published on: June 11, 2015

8.9K

Related Experiment Videos

Last Updated: Sep 14, 2025

Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System
05:19

Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System

Published on: June 28, 2024

987
Protein Engineering by Yeast Surface Display
05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

1.9K
Design and Construction of Artificial Extracellular Matrix aECM Proteins from Escherichia coli for Skin Tissue Engineering
10:30

Design and Construction of Artificial Extracellular Matrix aECM Proteins from Escherichia coli for Skin Tissue Engineering

Published on: June 11, 2015

8.9K

Area of Science:

  • Glycobiology and molecular cell biology.
  • Focus on the role of glycans in biological processes.

Background:

  • Glycans are critical regulators of cellular functions.
  • Characterizing glycan dynamics and structure-function relationships is essential.
  • Existing glycan-binding proteins (GBPs) have limitations in specificity and affinity.

Purpose of the Study:

  • To review recent advancements in engineering glycan-binding proteins (GBPs).
  • To outline strategies for developing next-generation GBPs for glycan analysis.
  • To address the gap between protein design principles and GBP engineering.

Main Methods:

  • Utilizing rational design principles for GBP engineering.
  • Employing directed evolution for optimizing lectin binding properties.
  • Exploring the modification of glycosyltransferases into novel GBPs.

Main Results:

  • Protein engineering can enhance GBP specificity and affinity.
  • Rational design and directed evolution are key strategies for GBP development.
  • Modifying glycosyltransferases offers a predictive approach to designing GBPs.

Conclusions:

  • Advanced protein engineering techniques are paving the way for novel GBPs.
  • Next-generation GBPs will enable deeper insights into the complex glycan landscape.
  • This work provides a roadmap for developing tailored glycan-binding tools.