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Updated: May 2, 2026
Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
Published on: January 5, 2015
An effective fluorescent sensor for lipopolysaccharide-induced H2S detection and imaging in inflammatory cells,
Xinrong Zou1,2,3,4, Meiling Pang5, Jun He5
1Department of Hematology, The Second Xiangya Hospital, Central South University Changsha Hunan 410011 PR China lingliestelle@csu.edu.cn linglimonica@aliyun.com.
Abstract:
In this work, a new fluorescent sensor (NAHCP) for the recognition of lipopolysaccharide-induced H2S detection and imaging in inflammatory Raw264.7 cells, zebrafish, and mouse blood samples was constructed. Based on the o-aldehyde group (o-CHO) auxiliary 2,4-dinitrophenyl ether (DNP) group sulfide cleavage, this sensor shows high sensitivity and selectivity towards H2S, which can realize fluorescence "turn-on" detection. This sensor also features a very high response speed (∼2 min) and a large Stokes shift (113 nm) toward H2S. Additionally, the excellent detection limit (LOD) for H2S is as low as 25.6 nM. Impressively, this o-CHO auxiliary DNP group sulfide cleavage ensemble is further successfully applied for H2S detection in inflammatory Raw264.7 cells, zebrafish, and mouse blood samples.
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