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Env-pseudoviruses based on the HIV-1 genetic variant circulating in Siberia
N B Rudometova1, A A Fando1, D N Shcherbakov1
1State Research Center Center of Virology and Biotechnology "Vector" Rospotrebnadzor, Koltsovo, Novosibirsk region, Russia.
Vavilovskii Zhurnal Genetiki I Selektsii
|July 23, 2025
Summary
Researchers developed new HIV-1 pseudoviruses from the CRF63_02A6 subtype, crucial for evaluating HIV vaccines and antivirals. This expanded collection aids in standardizing tests and advancing HIV prevention strategies globally.
Area of Science:
- Virology
- Immunology
- Vaccinology
Background:
- The global community faces ongoing challenges in controlling the Human Immunodeficiency Virus (HIV) pandemic, necessitating effective preventive vaccines and novel antiviral agents.
- Accurate evaluation of vaccine candidates, HIV-specific antibodies, and potential drugs requires reliable testing methods, such as pseudotype neutralization assays.
- Existing panels of Env-pseudoviruses represent key HIV-1 subtypes, but expansion is needed to cover circulating strains like CRF63_02A6 prevalent in Siberia.
Purpose of the Study:
- To expand and characterize a collection of Env-pseudoviruses based on the HIV-1 CRF63_02A6 recombinant form circulating in Siberia.
- To enhance the ability to standardize data for ranking vaccine effectiveness and identifying promising therapeutic candidates.
- To contribute to a comprehensive global panel for HIV-1 subtype characterization and drug/vaccine development.
Main Methods:
- Generation and characterization of two new Env-pseudovirus variants based on the HIV-1 CRF63_02A6 subtype.
- Phylogenetic analysis of full-length env gene nucleotide sequences to confirm clustering with reference CRF63_02A6 sequences.
- Neutralization assays using a panel of broadly neutralizing antibodies (bnAbs) targeting the HIV-1 Env glycoprotein.
Main Results:
- A collection of 13 CCR5-tropic Env-pseudoviruses based on CRF63_02A6 was established and characterized.
- Phylogenetic analysis confirmed all pseudoviruses belong to the CRF63_02A6 subtype.
- The pseudoviruses demonstrated varying sensitivity to bnAbs, being sensitive to VRC01 and 10E8, moderately sensitive to PG9 and PGT126, and resistant to 2G12 and 2F5.
Conclusions:
- The newly characterized Env-pseudoviruses based on HIV-1 CRF63_02A6 represent a valuable addition to existing global panels.
- This expanded collection facilitates standardized evaluation of vaccine candidates and antiviral agents against a key circulating HIV-1 subtype.
- The findings support the ongoing development of effective strategies to combat the HIV/AIDS pandemic.
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