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Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...

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Attomolar Nucleic Acid Detection Using CRISPR Enhanced Phase-Sensitive Surface Plasmon Resonance Imaging.

Xiaoqi Dai1, Changle Meng1, Songfeng Huang1

  • 1Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen 518060, China.

Analytical Chemistry
|July 23, 2025
PubMed
Summary

A new clustered regularly interspaced short palindromic repeats (CRISPR) enhanced Phase-interrogation Surface Plasmon Resonance imaging (CRISPR-PSPRi) sensor offers ultrasensitive, real-time nucleic acid detection. This advanced diagnostic tool can identify specific DNA from viruses like SARS-CoV-2 and monkeypox.

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Area of Science:

  • Biotechnology
  • Biosensing
  • Molecular Diagnostics

Background:

  • Traditional nucleic acid detection methods lack sensitivity and dynamic range for clinical diagnostics and pathogen surveillance.
  • Challenges include detecting low-concentration nucleic acids and single-nucleotide mutations, hindering real-time, amplification-free applications.

Purpose of the Study:

  • To develop an ultrasensitive, real-time, amplification-free, and label-free nucleic acid detection sensor.
  • To address limitations in sensitivity and dynamic range for detecting low-concentration DNA and single-nucleotide variations.

Main Methods:

  • Developed a clustered regularly interspaced short palindromic repeats (CRISPR) enhanced Phase-interrogation Surface Plasmon Resonance imaging (CRISPR-PSPRi) sensor.
  • Utilized CRISPR-Cas12a for target DNA recognition and trans-cleavage of ssDNA-linked gold nanoparticle probes.
  • Employed phase delay modulation and wavelength scanning for signal extraction and dynamic range extension.

Main Results:

  • Achieved high sensitivity (1.436 × 10-6 RIU) and a broad dynamic range (0.0111 RIU).
  • Successfully detected specific DNA from SARS-CoV-2 Omicron BA.2 variant and monkeypox virus.
  • Demonstrated detection of single-nucleotide mutations down to 1 aM concentration.

Conclusions:

  • The CRISPR-PSPRi sensor provides a real-time, high-throughput, and ultrasensitive nucleic acid detection platform.
  • This technology holds significant promise for advancing clinical diagnostics and pathogen monitoring.
  • Offers a viable solution for amplification-free and label-free detection of nucleic acids.