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Complete genome sequences of Mycobacterium smegmatis mc2155 cluster E bacteriophages Policronamos and Palpatine

Shule M Aggabao1, Rebecca Hassell1, Bryson S Leary1

  • 1Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, USA.

Microbiology Resource Announcements
|July 23, 2025
PubMed

Insights

Two mycobacteriophages, Policronamos and Palpatine, were analyzed. Their genomes show similarity to other phages in subcluster E, offering insights into mycobacterial phage diversity.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Mycobacteriophages are viruses that infect bacteria of the genus Mycobacterium.
  • Understanding mycobacteriophage diversity is crucial for developing novel therapeutic strategies against Mycobacterium infections.

Purpose of the Study:

  • To characterize the genomes of mycobacteriophages Policronamos and Palpatine.
  • To compare the genomic features of Policronamos and Palpatine with other known mycobacteriophages.

Main Methods:

  • Genome sequencing of mycobacteriophages Policronamos and Palpatine.
  • Bioinformatic analysis to identify open reading frames (ORFs) and assess gene synteny.

Main Results:

  • The genome of Policronamos is 75,894 bp with 146 ORFs.
  • The genome of Palpatine is 75,809 bp with 140 ORFs.
  • Gene synteny analysis revealed close resemblance to other phages within subcluster E.

Conclusions:

  • Mycobacteriophages Policronamos and Palpatine are distinct tailed phages infecting Mycobacterium smegmatis.
  • Their genomic characteristics place them within subcluster E, contributing to the broader understanding of mycobacteriophage evolution and classification.
Keywords:
bacteriophagephage

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