Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleosome Remodeling02:54

Nucleosome Remodeling

9.5K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
9.5K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.9K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.9K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

2.0K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.0K
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

5.5K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.5K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

2.8K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
2.8K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

2.8K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Innate Immunity of Framework Nucleic Acids.

Accounts of chemical research·2026
Same author

A multiple-encrypted DNA device for secure communication.

Science advances·2026
Same author

Visualized DNA Aptamer-Functionalized Silver Nanoclusters against Tuberculosis.

JACS Au·2026
Same author

Programming Dimensional Transitions in DNA Brick Crystals via Interfacial Connectivity.

Angewandte Chemie (International ed. in English)·2026
Same author

Structure-Controlled Molecular Recognition and Charge Transport in Metallized DNA Nanosheets.

Journal of the American Chemical Society·2026
Same author

Author Correction: Atomically precise photothermal nanomachines.

Nature materials·2026
Same journal

Carbonylative Aminative Suzuki-Miyaura Coupling: Pd-Catalyzed Synthesis of Amides from Vinyl/Aryl Halides and Boronic Acids.

Journal of the American Chemical Society·2026
Same journal

Divergent Asymmetric Synthesis of Glutinosasins A-E.

Journal of the American Chemical Society·2026
Same journal

Ultrastrong Polyketone Hot-Melt Adhesives Enabled by Ni-Catalyzed Carbonylative Polymerization.

Journal of the American Chemical Society·2026
Same journal

Programmable Anomalous Photovoltaics Enabled by Light-Electric Dual-Field Control.

Journal of the American Chemical Society·2026
Same journal

Biomimetic Redox-Mediated Proton Relay in Nanoreactors for Photocatalysis.

Journal of the American Chemical Society·2026
Same journal

The Sulfur Monoxide-Water Complex.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: Sep 14, 2025

Simple, Affordable, and Modular Patterning of Cells using DNA
08:59

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

4.3K

Dynamic Modulation of Multicellular Interactions via ATP-Dissipative DNA Assembly.

Yi Xu1,2, Yao Luo3, Xiaoyun Lu2

  • 1School of Automation and Intelligent Sensing, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

Journal of the American Chemical Society
|July 23, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed an ATP-dissipative DNA assembly system for dynamic cell control. This programmable system enables adaptive, reversible cell structures and enhances immunotherapy by modulating cell interactions.

More Related Videos

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
06:32

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Published on: March 9, 2022

1.9K
Mechanostimulation of Multicellular Organisms Through a High-Throughput Microfluidic Compression System
09:56

Mechanostimulation of Multicellular Organisms Through a High-Throughput Microfluidic Compression System

Published on: December 23, 2022

1.8K

Related Experiment Videos

Last Updated: Sep 14, 2025

Simple, Affordable, and Modular Patterning of Cells using DNA
08:59

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

4.3K
Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
06:32

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Published on: March 9, 2022

1.9K
Mechanostimulation of Multicellular Organisms Through a High-Throughput Microfluidic Compression System
09:56

Mechanostimulation of Multicellular Organisms Through a High-Throughput Microfluidic Compression System

Published on: December 23, 2022

1.8K

Area of Science:

  • Biomaterials Science
  • Synthetic Biology
  • Molecular Engineering

Background:

  • Living cells display dynamic adaptability via ATP-dependent processes vital for development and immunity.
  • Current cell assembly control methods lack the nonequilibrium and reversible nature of biological systems.

Purpose of the Study:

  • To engineer a novel ATP-dissipative DNA assembly system for spatiotemporal control of cell interactions.
  • To create adaptive, hierarchical cellular structures mimicking natural biological systems.

Main Methods:

  • Utilized programmable DNA monomers (dsDNA, tetrahedral DNA frameworks, branched DNA frameworks) for precise cell assembly.
  • Employed ATP-driven enzymatic reactions and liquid-liquid phase separation (LLPS) for dynamic control.
  • Applied the system to Ramos, PC-12, and natural killer (NK) cells, harnessing endogenous ATP.

Main Results:

  • Achieved precise, real-time, reversible control over cell assembly using the ATP-dissipative system.
  • Demonstrated dynamic modulation of intercellular interactions via branched DNA framework (BDF)-based condensates.
  • Showcased enhanced tumor-killing efficacy of NK cells through modulated interactions with cancer cells.

Conclusions:

  • The DNA-based dissipative self-assembly system offers precise spatiotemporal regulation of cellular interactions.
  • This approach holds significant potential for advanced applications in intelligent materials and immunotherapy.
  • The system's ability to harness endogenous ATP enables dynamic and reversible control of cellular behavior.