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Updated: Sep 14, 2025

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
Published on: April 30, 2011
Transcriptome-Wide Characterization of Protein:RNA Interactions with UV-Crosslinking Immunoprecipitation and
Manuel D Diaz-Muñoz1, Ines C Osma-Garcia2
1Toulouse Institute for Infectious and Inflammatory Diseases (INFINITy), Inserm UMR1291, CNRS UMR5051, University Paul Sabatier, CHU Purpan, Toulouse, France. manuel.diaz-munoz@inserm.fr.
Abstract:
The expansion of the cell genetic code relies on post-transcriptional regulatory mechanisms that shape the expression and translation of different mRNA transcripts into functional proteins. RNA-binding proteins (RBPs) control the splicing, editing, stability, and translation of mRNAs in a timely manner, and constitute an important layer of regulation that is often underestimated in genomic studies. Here we described individual nucleotide resolution UV-crosslinking immunoprecipitation and sequencing (iCLIP), a method that allows precise in vivo annotation of RBP-RNA interactions for identifying not only mRNA targets but also the mechanisms of action by which a given RBP controls the expression of single genes or gene-operons regulating cellular functions.
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