Protocol for conducting a single-cell sequencing assay for transposase-accessible chromatin analysis

Area of Science:

• Genomics
• Epigenetics
• Computational Biology

Background:

• Single-cell sequencing assay for transposase-accessible chromatin (scATAC-seq) offers single-cell resolution for chromatin accessibility studies.
• The analytical workflow for scATAC-seq can be challenging for new researchers.

Purpose of the Study:

• To provide a clear and accessible protocol for scATAC-seq analysis.
• To guide researchers through the entire analytical workflow, from pre-processing to multi-omics integration.

Main Methods:

• Demonstration of scATAC-seq analysis using a publicly available dataset.
• Detailed steps for data pre-processing and downstream analysis.
• Procedures for computational multi-omics integration.

Main Results:

• A comprehensive protocol for scATAC-seq data analysis.
• Facilitation of scATAC-seq adoption by researchers new to the technique.
• Enabling integrated analysis of multi-omics data.

Conclusions:

• This protocol effectively addresses the challenges associated with scATAC-seq analysis.
• It empowers researchers to confidently perform scATAC-seq data analysis and multi-omics integration.
• Facilitates deeper insights into chromatin accessibility at single-cell resolution.